Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization

Figure 3

B4 suppressed the dysfunction of MMP in LPS plus ATP-stimulated macrophage cells. (a, b) J774A.1 cells and THP-1 cells were pretreated with B4 for 4 h, then cocultured with LPS (1 μg/mL) for another 5 h, and stimulated with ATP (5 mM) for 30 min. The MMP levels were detected with JC-1 (10 μg/mL) for 30 min in an incubator in the dark. The images were captured by fluorescence microscopy. (c, d) The ratio of red fluorescence compared with green fluorescence in (a) and (b) was analyzed statistically by ImageJ; compared to the LPS+ATP group.

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