Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization

Figure 4

B4 blocked TLR4 dimerization. (a) The two plasmids TLR4-HA and TLR4-Flag were cotransfected into HEK293T cells for 24 h, then pretreated with B4 for 1 h, and cultured with LPS for 12 h. Cells were harvested, and Western blotting was used to analyze the formation of TLR4 dimerization. The experiments were performed three times independently. (b) The molecular docking data of B4 on TLR4-MD-2 dimer were analyzed by using LeDock software. The molecular docking data of B4 with TLR4-MD-2-LPS complex (PDB code: 3FXI) was performed in LeDock (http://www.lephar.com). TLR4-MD-2-LPS complex structures were obtained from the RCSB Protein Data Bank (PDB code: 3FXI). compared to the LPS group.

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