Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization

Figure 5

B4 rescued LPS-induced septic death and prevented LPS-induced ALI in mice. (a) Mice of experiment groups were treated with LPS (15 mg/kg, i.t.), and the B4-treated groups were injected with B4 (2.5, 5, and 10 mg/kg, i.v.) at 0, 3, 24, 48, and72 h after LPS administration. DEX group mice were injected with DEX (5 mg/kg, i.p.) after LPS treatment. The survival rate was recorded for the next 144 h (). (b–d) The mice were treated with LPS (4 mg/kg, i.t.). After LPS injection, mice were treated with B4 at 0, 3, 24, 48, and 72 h. After 72 h, blood samples were collected. Blood lymphocytes, neutrophils, and WBC were examined by the blood analyzer (). (e–g) The inflammatory cytokines TNF-α, IL-6, and IL-1β in serum were detected by ELISA (). (h) The MPO level in lung tissues was detected by MPO kit (). (i) H&E staining of the lung tissue (H&E, original magnification, 200x). DEX (5 mg/kg, i.p.) was used as a positive control (). , , and , compared to LPS-alone group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)