Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization

Figure 6

B4 rescued BLM-stimulated ALI in mice. (a) Mice were treated with BLM (3 mg/kg, i.t.). After BLM injection, mice were injected with B4 at 0, 3, 24, and 48 h. PFD group mice were administrated with PFD (300 mg/kg, i.g.) at 0, 3, 24, and 48 h after BLM treatment. Lung tissues were weighed, and lung index was calculated (). (b) Blood samples were collected. Blood neutrophils were examined by the blood analyzer, (). (c–e) The serum inflammatory cytokines TNF-α, IL-4, and TGF-β1 were detected by ELISA (). (f) H&E staining of the lung tissue (H&E, original magnification, 200x). PFD (300 mg/kg, i.p.) was the positive control (). , , and , compared to the BLM-alone group.

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