Effects of miR-373 Inhibition on Glioblastoma Growth by Reducing Limk1 In Vitro

Figure 2

Inhibition of miR-373 reduced the U-251 cell proliferation. (a) Anti-miR-373 could reduce the U-251 cell proliferation by MTT assay. (b) PCNA mRNA level was also reduced after anti-miR-373 transfection by real-time quantitative PCR in U-251 cells. (c) Western blot results proved the lower expression of PCNA protein after anti-miR-373 transfection in U-251 cells. (d) After ionizing radiation (IR), the cell number of U-251 cells was reduced obviously by MTT assay, and additional effects were also obtained in the group of IR combined with anti-miR-373 transfection. (e) IR could also reduce the PCNA mRNA level in U-251 cells, and such inhibition was enhanced after being combined with anti-miR-373 transfection. (f) PCNA protein expression was also reduced significantly after ionizing radiation in the presence or absence of anti-miR-373 transfection. (g) After TMZ treatment, the cell number of U-251 cells was reduced obviously by MTT assay, and additional effects were also obtained in the group of TMZ treatment in the presence of anti-miR-373 transfection. (h) TMZ could also reduce the PCNA mRNA level in U-251 cells, and such inhibition was enhanced after being combined with anti-miR-373 transfection. (i) PCNA protein expression was also reduced significantly after TMZ treatment in the presence or absence of anti-miR-373 transfection (bars indicate the standard deviation of the mean; each experiment was performed in triplicate; ; ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)