Successful Regulatory T Cell-Based Therapy Relies on Inhibition of T Cell Effector Function and Enrichment of FOXP3+ Cells in a Humanized Mouse Model of Skin Inflammation

Figure 2

Treg infusion inhibits PBMC-induced skin inflammation in vivo. Representative histology at 10x magnification and quantitative analysis of human skin grafts from SCID beige mice at 21 days after cell injection of PBMC with or without ex vivo-expanded Treg. The white line indicates the border of the epidermis. The inlay at (b) PBS and (d) PBMC shows 20x magnification: (a) epidermal thickness (μm, HE staining). Parakeratosis is pointed by blue arrows and human cell infiltration by red arrows, and a microabscess is indicated by an asterisk. (b–d) Epidermal expression patterns of K10, K16, and hBD2. (e) Bar plots show the percentages of the positive area of (a–d), and (b–d) show the percentage of positive cells within the epidermis. PBS (), PBMC (), and PBMC+Treg (). Statistical significance was analysed by the Mann–Whitney test. value < 0.05, value < 0.01, and value < 0.001.