Successful Regulatory T Cell-Based Therapy Relies on Inhibition of T Cell Effector Function and Enrichment of FOXP3+ Cells in a Humanized Mouse Model of Skin Inflammation

Figure 4

Treg infusion affects systemic proinflammatory cytokine production by T cells. Representative flow cytometry pictures and quantitative analysis of systemic human CD45+ and CD3+ T cells harvested from the mouse spleen of SCID beige mice infused with PBMC with or without Treg. (a) Percentage of human CD45 cells. (b) Percentage of human CD4+ and CD8+ cells within human CD45+ cells. (c) Representative example of the percentages of dividing (Ki67+) CD4+ and CD8+ cells (). (d) Percentage of human IFNγ and IL-17A-secreting T cells. (e) Frequency of CD4+CD25+FOXP3+ cells within CD45+ cells and Treg : CD4 ratio analysis. is shown for (a, b, d) (). Statistical significance was analysed by the Mann–Whitney test. value < 0.05; value < 0.01.

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