Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice

Figure 6

Inhibition of UBA1 blunted ox-LDL-induced lipid accumulation and expression of proinflammatory cytokines and NADPH oxidases in cultured macrophages. (a) Oil red O staining of ox-LDL-treated RAW264.7 cells. (b) Quantitative real-time PCR analysis of proinflammatory (Il-1β, Il-6, and Tnfα) and anti-inflammatory (Il-4 and Il-10) cytokine expression in ox-LDL-treated RAW264.7 cells. (c) Quantitative real-time PCR analysis of NADPH oxidase (Nox1, Nox2, and Nox4) expression in ox-LDL-treated RAW264.7 cells. per group; : <0.05; : <0.01; ns: not significant.

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