Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia

Figure 1

IL-33 and tissue-resident ILC2s in lung increased markedly in BPD mice. (a) BPD C57BL/6 mice model was established with lipopolysaccharide (LPS) using pregnant mice at 16.5 days of gestation. Neonatal mice were euthanized for lungs on P1, P3, P7, P14, and P21. (b) Representative hematoxylin-eosin-stained sections of the lung from C57BL/6 mice treated with saline or LPS. Original magnification, ×200. (c) Representative FACS analysis shows the gating strategy to identify Lin^-CD90.2⁺Sca-1⁺ILC2 in mouse lung. (d) Representative results of ILC2s detected by flow cytometry on different postnatal days in saline (control) and LPS (BPD) groups. (e) Percentage of ILC2 in the lung on different postnatal days in saline and LPS groups. (f) The expression of IL-33 in the lung on different postnatal days in saline and LPS groups. Data are represented as . . The LPS group was compared with the saline group at the same time point, and indicates , indicates , and indicates .

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