Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia

Figure 5

Downregulation of ILC2s involved in the regulation of DEX to alleviate BPD. (a) C57BL/6 BPD mice were treated with DEX or PBS daily from day 7 after birth. Neonatal mice were euthanized for lungs on P14. (b) Representative hematoxylin-eosin-stained sections of the lung from C57BL/6 mice treated with saline, LPS+vehicle (PBS) and LPS+DEX. Original magnification, ×200. (c) Representative results of ILC2s detected by flow cytometry on different postnatal days in saline, LPS+vehicle, and LPS+DEX groups. (d) Percentage of ILC2s in the lung on different postnatal days in saline, LPS+vehicle, and LPS+DEX groups. (e, f) Relative expression of IL-4 and IL-13 in the lung in saline, LPS+vehicle, and LPS+DEX groups, respectively. (g, h) Representative results of M1 and M2 macrophages detected by flow cytometry on different postnatal days in saline, LPS+vehicle, and LPS+DEX groups, respectively. (i) Percentage of M1 macrophages in the lung on different postnatal days in saline, LPS+vehicle, and LPS+DEX groups. (j) Percentage of M2 macrophages in the lung on different postnatal days in saline. Data are represented as . . indicates ; indicates .

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