Development and Validation of a Bordetella pertussis Whole-Genome Screening Strategy

Figure 1

Original childhood aP priming is associated with high reactivity to pertussis antigens. (a) Cytokine production was measured by ELISpot following in vitro restimulation with BP lysate. Magnitude of responses expressed by spot-forming cells (SFC) is shown for the sum of T cell responses (IFNγ, IL-5, IL-17, and IL-9) for all aP antigens (PT) or ACT between wP- and aP-primed donors ( for aP and for wP cohorts). (b) % of BP-specific CD4+ memory T cells for PT or ACT antigens by AIM assay for donors originally primed with wP or aP vaccine ( for aP and for wP cohorts). (c) Differential polarization of T cell responses for PT but not ACT. Each data point represents the ratio of IFNγ/IL-5 SFCs from each donor ( for aP and for wP cohorts). Data are expressed as for each cohort with the Mann–Whitney test comparison value.

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