Development and Validation of a Bordetella pertussis Whole-Genome Screening Strategy

Figure 5

Ex vivo AIM assay pool screening on different days reproducibly identifies candidates for deconvolution. (a) % of BP-specific CD4+ T cells for the pool of aP antigens (PT) or for the 8 MSs containing peptides from the 4 individual aP antigens was measured by the AIM assay. Peptides from the 4 individual antigens are aligned by ORF position but flanked by peptides from other antigens and unequally distributed over 2 adjacent and unique MSs each. Individual circles represent an independent technical replicate of the same donor performed on a different day. A representative donor is shown. Data are expressed as . Dotted line indicates positive response threshold of 100 cells set to identify MS candidates for deconvolution. (b) Memory subset composition of PT MG- and MS-specific responses () is shown as % of total CD4+ T cells gated in AIM-positive cells (Tn: CD45RA+CCR7+, Temra: CD45RA+CCR7-, Tcm: CD45RA-CCR7+, and Tem: CD45RA-CCR7-). The average data for each positive mesopool are shown ().

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