Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation

Figure 3

Effects of thalidomide on iNOS, CD206, Arg-1 and TNF-α protein expression in 33.3 mM glucose-induced macrophage. (a) iNOS and CD206 protein expressions. (d) Arg-1 protein expression. (f) TNF-α protein expression. The results of iNOS, CD206, Arg-1, and TNF-α were represented in (b), (c), (e), and (g), respectively. All results were expressed as a ration with respect to control and represented as the in triplicates. ; versus 11.1 mM glucose. ^#; versus 33.3 mM glucose. ^&; versus Tha100. ^{^}; versus Tha50. Abbreviations: LPS: lipopolysaccharide; Tha50: 50 μg/ml thalidomide in 33.3 mM glucose; Tha100: 100 μg/ml thalidomide in 33.3 mM glucose; Tha200: 200 μg/ml thalidomide in 33.3 mM glucose; iNOS: inducible nitric oxide synthase; CD206: mannose receptor; TNF-α: tumor necrosis factor-α; Arg-1: arginase-1.

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