Tumor-Infiltrating Immune Cells and PD-L1 as Prognostic Biomarkers in Primary Esophageal Small Cell Carcinoma

Figure 2

CD4⁺ TILs colocalize with PD-L1⁺CD163⁺ TAMs. Representative multicolor immunofluorescence (multi-IF) images showing (a) high and (b) low colocalization of PD-L1⁺ cells with CD163⁺ TAMs. Scale bar, 100 μm. Representative multi-IF images showing costaining of (c) CD4⁺PD-L1⁺CD163⁺ TILs and (d) CD8⁺PD-L1⁺CD163⁺ TILs in serial sections of the same specimen. Cells were counterstained with DAPI (blue, nucleus). Scale bar, 100 μm.

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