Identification and Functional Analysis of EPOR⁺ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis

Figure 7

EPO increased the expression of M2 cytokines. (a) Western blot analysis showing phosphorylation of STAT5 and AKT in primary osteosarcoma macrophages upon EPO stimulation. (b) The mRNA expression of IL-10, TGFβ1, VEGFA, and IGF-1 on osteosarcoma macrophages upon EPO stimulation. (c) The protein expression of IL-10, TGFβ1, VEGFA, and IGF-1 on osteosarcoma macrophages upon EPO stimulation.

(a)

(b)

(c)