Elemental Diet Enriched with Amino Acids Alleviates Mucosal Inflammatory Response and Prevents Colonic Epithelial Barrier Dysfunction in Mice with DSS-Induced Chronic Colitis

Figure 3

An elemental diet enriched in amino acids affects the accumulation of intestinal lamina propria dendritic cells (DCs) in chronic colitis mice. Mouse splenic mononuclear cells (MNCs), mesenteric lymph nodes (MLNs), and colonic lamina propria mononuclear cells (LPMCs) were isolated from individual groups of mice. Representative flow cytometric analyses of CD11C⁺CD103⁺CD11b⁺ DCs (a); MNCs were gated in CD11C⁺, and the percentages of CD103⁺CD11b⁺ DCs were analyzed in the spleen; MLNs and LPMCs in different groups of mice (b). Representative flow cytometric analyses of CD3⁻ NK1.1⁺ NK cells (c) and NK proportions in MNCs (d) in the spleen, MLN, and LPMCs of different groups of mice ( per group). Data are representative images or expressed as the of each group from three separate experiments. , , ; ns: not significant.

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