Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping

Figure 3

Signal analysis of IgY-C against the SARS-CoV-2 Proteome Microarray. A peptide microarray was performed with IgY-C (10 μg/ml), and the resulting fluorescence intensities were plotted against sequential peptides covering SARS-CoV-2 S protein from N- to C-terminus. The fluorescence intensity plot of IgY-S (10 μg/ml) was incorporated for comparison. Weak signal peaks from peptide spots not of “epitope signal pattern” can be seen, and some of these peptides contain an N-terminal TD motif. Signal peaks corresponding to motif EIL are present for both IgY-S and IgY-C.