Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping

Figure 4

IgY-S epitope mapping against the SARS-CoV Antigen Microarray. Peptide microarrays were performed with IgY-S at 1 μg/ml and 10 μg/ml, and the resulting fluorescence intensities were plotted in relation to sequential peptides covering SARS-CoV-2 S protein from N- to C-terminus. Fluorescence intensity peaks corresponding to the consensus motifs IVAYTMSLG and VDLGDISGI as well as an EIL-like motif are indicated. Weak signal peaks from peptide spots not of “epitope signal pattern” are also shown.