Knockdown of YAP/TAZ Inhibits the Migration and Invasion of Fibroblast Synovial Cells in Rheumatoid Arthritis by Regulating Autophagy

Figure 5

Knockdown of YAP or TAZ promoted autophagic activity in RA-FLS. (a) Quantitative analysis of mRNA levels of autophagy-related genes in stable YAP or TAZ knockdown RA-FLS by RT-qPCR and normalized against GAPDH. Data were shown as the (). relative to shC by Student’s -test. (b) Protein levels of ULK1, LC3B, and p62 were determined by western blot in stable YAP or TAZ knockdown RA-FLS, and GAPDH was used as a loading control. Representative blots are shown (). (c) LC3B puncta detected by immunofluorescence staining assay in stable YAP or TAZ knockdown RA-FLS (). Magnification, ×1000.

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