Duck Enteritis Virus VP16 Antagonizes IFN-β-Mediated Antiviral Innate Immunity

Figure 1

The immune evasion of DEV in vitro. (a) DEFs were cultured in 6-well plates and infected with WT DEV (1 MOI) when they grew up to 90%. At 4 hours, 12 hours, and 24 hours, the infected cells were collected and real-time qPCR was performed to determine the transcriptional levels of duck IFN-β in the DEF. (b) DEFs were seeded in 24-well plates and transfected with 500 ng of the duIFN-β-Luc (duck IFN-β promoter reporter plasmid), together with 50 ng of pRL-TK (Renilla luciferase plasmid, Promega, America) and pCAGGS empty vector or plasmids encoding the indicated protein (duck IRF7, duck STING, or duck cGAS, 500 ng/well). Cells were mock infected or infected with 0.5 MOI WT DEV 12 hours posttransfection, and firefly luciferase activities were measured at 48 h postinfection; the data were analyzed by GraphPad Prism software, and results were presented using two-way ANOVA () and considered significant ().

(a)

(b)