Duck Enteritis Virus VP16 Antagonizes IFN-β-Mediated Antiviral Innate Immunity

Figure 5

DEV VP16 associates with duck IRF7 but not with duck IRF1. HEK293T cells were cotransfected with pCAGGS-UL48-HA and pCAGGS-IRF7-Flag or pCAGGS-IRF1-Flag. (a) At 48 hours after transfection, cells were resolved to coimmunoprecipitation and immunoblot analysis with the indicated antibodies. (b) Colocalization of UL48 (green) and IRF7 (red) in HEK293T cells, the nuclei were stained by DAPI (1 : 1000) (blue). (c) Localization of UL48 (green) and IRF1 (red) in HEK293T cells. Nuclei stained by DAPI were shown in blue.

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