Duck Enteritis Virus VP16 Antagonizes IFN-β-Mediated Antiviral Innate Immunity

Figure 6

N-terminal of VP16 plays the most effective role in the inhibition of IFN-β promoter activity. (a) Construction of different kinds of truncated VP16 protein. (b, c) Transfection of DEFs that were cultured in 24-well plates with duIFN-β-Luc (500 ng), pRL-TK (50 ng), and VP16 or plasmids encoding truncated VP16, the same with that described in Figure 2(a).

(a)

(b)

(c)