Dendritic Cells Transfected with MHC Antigenic Determinants of CBA Mice Induce Antigen-Specific Tolerance in C57Bl/6 Mice

Figure 6

Induction of GVHD in BALB/c mice by splenocytes cocultured with transfected DCs. (a) Spleen weight of BALB/c host mice 3 weeks after GVHD induction. (b) FoxP3⁺ Treg frequencies in host mouse spleens 3 weeks after GVHD induction. per group. Median and interquartile range. Brackets indicate significant differences (the Kruskal-Wallis test with Dunn’s corrected multiple comparisons, ). Saline: administration of saline; Spl: administration of C57BL/6 mouse splenocytes; DCp5: administration of cocultures of autosplenocytes and DCs electroporated with a control plasmid p5; DCpIL-10: administration of cocultures of autosplenocytes and DCs electroporated with a pIL-10; DCpMHC: administration of cocultures of autosplenocytes and DCs electroporated with a pMHC; DCpIL-10⁺MHC: administration of cocultures of autosplenocytes and DCs electroporated with pMHC and pIL-10 simultaneously.

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