Dendritic Cells Transfected with MHC Antigenic Determinants of CBA Mice Induce Antigen-Specific Tolerance in C57Bl/6 Mice

Figure 7

Suppression of allogeneic skin graft rejection by splenocytes cocultured with transfected DCs. Experimental design (a) and survival curves for (b) CBA and (c) BALB/c skin grafts. (b) . (c) . The median graft survival time has indicated in round brackets for each group, where applicable. Square brackets indicate significant differences (the Mantel-Cox test and log-rank test for trend, ). Syngeneic: transplantation of syngeneic skin graft without other interventions (positive control); Saline: administration of saline; non-EP DC: administration of nonelectroporated DCs; DCp5: administration of cocultures of autosplenocytes and DCs electroporated with a control plasmid p5; DCpIL-10; administration of cocultures of autosplenocytes and DCs electroporated with pIL-10; DCpMHC: administration of cocultures of autosplenocytes and DCs electroporated with pMHC; DCpIL-10⁺MHC; administration of cocultures of autosplenocytes and DCs electroporated with pMHC and pIL-10 simultaneously.

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