Endometrial Cancer Cells Promote M2-Like Macrophage Polarization by Delivering Exosomal miRNA-21 under Hypoxia Condition

Figure 3

Hypoxic KEL cell-derived exosomes horizontally transferred miRNA-21 to the THP-1 cells. THP-1 cells were treated with exosomes labeled with PKH26 for 12 h, and after that, exosomes were washed off and media replaced. Nuclei were stained with DAPI. miRNA-21 levels were identified in THP-1 cells using RT-PCR. U6 was used to normalize the Ct values between the samples. (a) Representative fluorescence microscopy of THP-1 after incubation with exosomes. (b) Statistical assay of the expression level of miRNA-21 in THP-1 after the uptake of exosomes. N: normoxia; Hx: hypoxia.

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