Endometrial Cancer Cells Promote M2-Like Macrophage Polarization by Delivering Exosomal miRNA-21 under Hypoxia Condition
Immunomodulatory effect of exosomes produced by KEL cells on human THP-1 monocytes under hypoxia condition. THP-1 cells were cocultured with exosomes excreted by KEL cells under hypoxia or normoxic condition for 24 h. Then, mRNA or protein expression levels of TNF-α, IL-1β, IL-10, and CD206 were detected. (a) The statistical assay of mRNA expression of TNF-α, IL-1β, IL-10, and CD206 in THP-1 cells. (b) ELISA assayed the protein levels of TNF-α, IL-1β, and IL-10 in the supernatants. The results represent three independent experiments. N: normoxia; Hx: hypoxia. ,.
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