Endometrial Cancer Cells Promote M2-Like Macrophage Polarization by Delivering Exosomal miRNA-21 under Hypoxia Condition

Figure 5

KLE-cell derived exosomal miRNA-21 promoted M2-like polarization of THP-1 monocytes under hypoxia condition. (a) miRNA-21 mimics or negative control was transfected into THP-1 cells for 24 h. The statistical assay of TNF-α, IL-1β, IL-10, and CD206 mRNA expression in THP-1 cells. (b) miRNA-21 inhibitor and negative control were introduced to the THP-1 cells and then cocultured with exosomes derived from hypoxic KEL cells. The statistical assay of TNF-α, IL-1β, IL-10, and CD206 mRNA expression in THP-1 cells. (c) The experiment is the same as (a). The statistical assay of the protein levels of TNF-α, IL-1β, and IL-10. (d) The experiment is the same as (b). The statistical assay of the protein levels of TNF-α, IL-1β, and IL-10 in the supernatants. The results represent three independent experiments. , .

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