Remote Inflammatory Preconditioning Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Inhibition of Intrinsic Apoptosis in Rats

Figure 2

RInPC prevented apoptosis via an intrinsic pathway in LPS-induced ALI in rats. (a) Fluorescent images of the TUNEL results under a fluorescence microscope in ALI rats induced by LPS (original magnification: 400x). (b) Comparison of apoptosis index between each group. (c) WB bands of caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, Bcl-2, and Bax. (d) Comparison of caspase-3, cleaved caspase-3, caspase-9, and cleaved caspase-9 between each group. (e) Comparison of Bcl-2, Bax, and Bcl-2/Bax ratio between each group. (f) WB bands of Cyt-c and AIF expressed in the cytoplasm and mitochondria. (g) Comparison of Cyt-c and AIF in the cytoplasm and mitochondria between each group. ; vs. control; ^##; ^### vs. LPS (). DAPI: 4,6-diamidino-2-phenylindole; WB: western blot; Cyt-c: cytochrome c; AIF: apoptosis-inducing factor; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; COX4: cytochrome c oxidase subunit 4.

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