Research Article
Supplemental Bacillus subtilis PB6 Improves Growth Performance and Gut Health in Broilers Challenged with Clostridium perfringens
Table 2
Sequences for real-time PCR primers.
| | Genes | Primer sequence (5–3) | Accession no. |
| | β-Actin | F: GAGAAATTGTGCGTGACATCA
R: CCTGAACCTCTCATTGCC | L08165 | | Occludin | F: ACGGCAGCACCTACCTCAA
R: GGGCGAAGAAGCAGATGAG | D21837.1 | | ZO-1 | F: CTTCAGGTGTTTCTCTTCCTCCTC
R: CTGTGGTTTCATGGCTGGATC | XM_413773 | | TRL4 | F: GTTCCTGCTGAAATCCCAAA
R: TATGGATGTGGCACCTTGAA | NM_001030693 | | IL-1β | F: ACTGGGCATCAAGGGCTA
R: GGTAGAAGATGAAGCGGGTC | NM_204524 | | TNF-α | F: GAGCGTTGACTTGGCTGTC
R: AAGCAACAACCAGCTATGCAC | NM_204267 | | IFN-γ | F: TAACTCAAGTGGCATAGATGTGGAAG
R: GACGCTTATGTTGTTGCTGATGG | NM_008337 |
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1Abbreviation: ZO-1: zonula occludens-1; TRL4: Toll-like receptor-4; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-α; IFN-γ: interferon-γ.
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