Research Article
Short-Chain Fatty Acids Promote Immunotherapy by Modulating Immune Regulatory Property in B Cells
Figure 3
PA stabilizes IL-10 mRNA in human B cells. B cells were isolated from blood samples collected from HC subjects. (a) Violin plots show IL-10 protein levels in culture supernatant after exposing to CpG (1 μg/ml) for 16 h (the 1st stage). (b) The curve shows IL-10 mRNA levels in B cells after a 5 h culture (the 2nd stage) in fresh medium in the presence or absence of PA following the exposure to CpG in the culture for 16 h. (c) Violin plots show IL-10 mRNA levels in B cells after the treatment denoted on the axis at different time points. PA: 10 μM; GPR41d (GPR43d): GPR41-deficient (or GPR43-deficient) B cells (prepared by RNAi); Control: B cells were treated with scrambled RNAi. ( test for (a); ANOVA+Dunnett’s test for (c)), compared with the saline group. Each dot in the violin plots presents data obtained from one experiment.
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