Short-Chain Fatty Acids Promote Immunotherapy by Modulating Immune Regulatory Property in B Cells

Figure 6

YPS induces PA production by intestinal bacteria to increase CD5⁺ B cells in the mouse airway tissues. AR mice and NC mice were treated with the procedures listed above the FACS plots (a). YPS: mice were gavage-fed with YPS daily during sensitization; Bug.d: mice were gavage-fed with antibiotic cocktail to deplete intestinal bacteria; IPA: mice were fed with indole-PA-3 daily during sensitization. (a) Gated FACS plots show CD5⁺ B cell counts in airway mononuclear cells (AMCs). (b) CD5⁺ B cell frequency in AMCs. (c) Mouse serum PA levels. (d, e) Total RNA and proteins were extracted from CD5⁺ B cells (purified from AMCs and with equal cell number per sample ( cells/sample)). Boxplots show IL-10 mRNA levels in airway CD5⁺ B cells. Each group consists of 6 mice. Each dot in boxplots presents data obtained from one sample. (ANOVA+Dunnett’s test), compared with the NC group. ^## and ^### (t test), compared with group c.

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