Stress Induces Release of Extracellular Vesicles by Trypanosoma cruzi Trypomastigotes

Figure 2

EV release (particles/mL), size (nm), and membrane integrity under chemical stress. Trypanosoma cruzi trypomastigotes were incubated in the absence or presence of methyl-beta-cyclodextrin (CBD) (25 μg/mL) or NaN₃ (0.0001 to 1%) for 2 h at 37°C. EVs in the supernatant were quantified by NTA in triplicate (). The panels show EV concentrations (a, b) and size (nm) (c) in the presence of chemical agents. SEM of trypomastigotes preincubated with the indicated concentrations of NaN₃ (d–g). Size bars are defined in each image. The relative fluorescence (RFU) of PrestoBlue viability reagent (, ) is shown in panel (h).

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