Stress Induces Release of Extracellular Vesicles by Trypanosoma cruzi Trypomastigotes

Figure 3

pH effect on EV release. Trypomastigote (/mL) forms incubated (2 h, 37°C) in culture medium containing 5% glucose previously adjusted to the indicated pH values. EV concentrations (a) and sizes (b) were analyzed by NTA (). After incubation at pH 5 (c) or pH 7 (d), parasites were analyzed by SEM. In parallel, parasites were incubated at pH 5 (e) or pH 7 (f), stained with Giemsa, and 300 parasites were evaluated according to their form: trypomastigotes (Trypos), intermediate (Inter), or amastigote (Amas). Their percentages were quantified in triplicate.

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