Human Adenovirus Serotype 3 Infection Modulates the Biogenesis and Composition of Lung Cell-Derived Extracellular Vesicles

Figure 1

The effect of HAdV3 on A549 viability. (a) Intracellular esterase activity of viable A549 cells. Cells were labeled with nonfluorescent calcein-AM dye which is converted to green fluorescent molecules as a result of ester hydrolysis. The images were acquired at the indicated time after incubation. (b) Postinfection quantification of viable A549 cells after incubation with 3-(4,5-dimethylthiazol-2-yl)-25-diphenyltetra bromide after 24 h (c) 48 h, and (d) 72 h. The cells were incubated with dye solution at 37°C for 3-4 h; absorbance was read at 570 nm. Data shows the from four independent experiments performed using one-way analysis of variance (ANOVA) with Tukey post hoc analysis. Statistical significance is indicated by the as follows: , , , and .

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