Identification of a Prognosis-Related Risk Signature for Bladder Cancer to Predict Survival and Immune Landscapes

Figure 7

Experiment of TNFRSF14. (a) Relative RNA level of TNFRSF14 in T24 and UMUC3 cells following TNFRSF14 knockdown. GAPDH served as loading control. (b) Relative protein level of β-catenin and cyclin D1 in T24 and UMUC3 cells following TNFRSF14 knockdown. β-Tubulin served as loading control. (c) The effects of TNFRSF14 on the proliferation of T24 and UMUC3 cells were analyzed by CCK-8 assays. The results are presented as the mean optical density (OD) at 450 nm for triplicate wells. The results are presented as the of three independent experiments (; ). (d) EdU incorporation assays were used to determine the effects of TNFRSF14 on T24 and UMUC3 cell proliferation. The ratio of EdU-positive cells (green) per field to the number of Hoechst 33342-positive cells (blue) in the same field was calculated in three random fields.

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