Yangxue Jiedu Fang Ameliorates Psoriasis by Regulating Vascular Regression via Survivin/PI3K/Akt Pathway

Figure 7

The effect of YXJD on HUVEC cells induced by Survivin and its molecular mechanism HUVEC cells transfected with Survivin overexpressed plasmid were treated with different concentrations of YXJD (0, 1, and 4 mg/mL) for 24 h by CKK-8 detection (a). The induction of apoptosis was determined by annexin V–FITC/PI staining assay (b, c). Transwell: the effect of different concentrations of YXJD (0, 1, and 4 mg/mL) on the migration ability of HUVEC cells transfected with Survivin overexpression plasmid (d). Statistical chart: ordinate: number of cells (e). Tube formation detection was used to detect the effects of different concentrations of YXJD on the angiogenic ability of HUVEC cells transfected with Survivin overexpression plasmid (f). Meshes number (g) and master segments length (h). Statistical chart: ordinate: number of lumen/length of lumen. Magnification: 100× (f–h). Flow cytometry cell detected cycle distribution of HUVEC cells transfected with Survivin overexpression plasmid with different concentrations of YXJD (i). Statistical chart: ordinate: cell number (j).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)