Yangxue Jiedu Fang Ameliorates Psoriasis by Regulating Vascular Regression via Survivin/PI3K/Akt Pathway

Figure 8

Western blot: the effect of different concentrations of YXJD on the expression of Survivin (a), LC3II/I, and p62 (d) in HUVEC cells transfected with Survivin overexpression plasmid, and the expression of PI3K/Akt- β catenin pathway and Caspase3 (e). ELISA: the effect of different concentrations of YXJD (0, 1, and 4 mg/mL, for 72 h) on the secretion of VEGF in HUVEC cells transfected by Survivin overexpression plasmid (b). qRT-PCR: the effect of different concentrations of YXJD on the synthesis of VEGF in HUVEC cells transfected by Survivin overexpression plasmid (c). GAPDH was used as the internal reference for cytoplasmic protein content and total protein content; Laminb as the internal reference for nuclear protein content; total stands for total protein; cytoplasm for cytoplasmic protein; nucleus for nuclear protein.

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