Protective Effect of Uric Acid on ox-LDL-Induced HUVECs Injury via Keap1-Nrf2-ARE Pathway

Figure 1

HUVEC injury caused by ox-LDL stimulation at different concentrations HUVECs were stimulated with 0, 10, 20, 50, 100, and 200 μg/mL ox-LDL for 24 h. Changes in (a) cell morphology in different groups. (b) Cell survival rate in different groups by CCK-8 assay.  μm. (c) Changes in MDA levels. (d, e) DHE immunofluorescence image and densitometry analysis of immunofluorescent intensity of DHE.  μm. (f, g) ICAM1 immunofluorescence image and densitometry analysis of immunofluorescent intensity of ICAM1.  μm. DHE: dihydroethidium; HUVECs: human umbilical vein endothelial cells; ox-LDL: oxidized low-density lipoprotein; MDA: malondialdehyde. The statistical analyses were done using GraphPad Prism V-7.0 software. Data are expressed as the (). One-way ANOVA was used in statistical analyses (/group). , , , and versus the control.

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