Protective Effect of Uric Acid on ox-LDL-Induced HUVECs Injury via Keap1-Nrf2-ARE Pathway

Figure 2

Changes of HUVECs under the stimulation of different concentrations of uric acid HUVECs were stimulated with 0, 1, 2, 3, 5, 6, 9, 12, 15, and 18 mg/dL uric acid for 24 h. Changes in (a) cell morphology in different groups.  μm. (b) Cell survival rate in different groups by CCK-8 assay. (c, d) DHE immunofluorescence image and densitometry analysis of immunofluorescent intensity of DHE.  μm. (e, f) ICAM1 immunofluorescence image and densitometry analysis of immunofluorescent intensity of ICAM1.  μm. DHE: dihydroethidium; HUVECs: human umbilical vein endothelial cells. The statistical analyses were done using GraphPad Prism V-7.0 software. Data are expressed as the (). One-way ANOVA was used in statistical analyses (/group). , , , and versus the control.

(a)

(b)

(c)

(d)

(e)

(f)