Protective Effect of Uric Acid on ox-LDL-Induced HUVECs Injury via Keap1-Nrf2-ARE Pathway

Figure 3

Changes of HUVECs under the stimulation of different concentrations of uric acid and fixed concentration ox-LDL. HUVECs were pretreated with 0, 1, 2, 3, 5, 6, 9, 12, 15, and 18 mg/dL uric acid 2 h before 100ug/mL ox-LDL stimulated. Changes in (a) cell morphology in different groups.  μm. (b) Cell survival rate in different groups by CCK-8 assay. (c) MDA level in different groups. (d, e) DHE immunofluorescence image and densitometry analysis of immunofluorescent intensity of DHE.  μm. (f, g) ICAM1 immunofluorescence image and densitometry analysis of immunofluorescent intensity of ICAM1.  μm. DHE: dihydroethidium; HUVECs: human umbilical vein endothelial cells; ox-LDL: oxidized low-density lipoprotein; MDA: malondialdehyde. The statistical analyses were done using GraphPad Prism V-7.0 software. Data are expressed as the (). One-way ANOVA was used in statistical analyses (/group). , , , and versus the control.

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