Protective Effect of Uric Acid on ox-LDL-Induced HUVECs Injury via Keap1-Nrf2-ARE Pathway

Figure 6

Effects of uric acid in microfluidic devices on inflammation and oxidative stress caused by ox-LDL in HUVECs. (a) Schematic diagram of vascular endothelial cells. (b) Photograph of a prototype. (c) A schematic diagram of cells in the micro chambers. (d) The microfluidic chambers were perfused with medium containing red THP-1 cells at velocity of 5 μL/min. HUVECs were stained by Mito-Green, determined by green fluorescence.  μm. (e) The mRNA levels of TGFβ, TNFα, IL-1β, IL-6, and Nox4 were assessed by reserve transcription PCR. GAPDH served as loading controls. Group data were obtained by normalizing to GAPDH and expressed as fold of control values. The statistical analyses were done using GraphPad Prism V-7.0 software. Data are expressed as the (). One-way ANOVA was used in statistical analyses (/group). , , , and versus the control. ^#, ^##, ^###, and ^#### versus the ox-LDL group.

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