Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development

Figure 5

miR-136 is a direct target of circ_0044516. (a) miR-136 expression in 20 pairs of lung cancer tissues. (b) RNA pull-down assays were carried out using biotin-miR-136, control, or 10% input. (c) Predicted WT or MUT miR-136 binding sites in circ_0044516. (d) Relative luciferase activities were measured in A549 and SPCA1 cells cotransfected with circ_0044516-WT or circ_0044516-MUT and miR-136 mimics or miR-NC by luciferase reporter gene assays. (e) Expression of miR-136 in lung cancer cells transfected with circ_0044516 siRNA or circ_0044516 overexpression plasmid by RT-qPCR. Data are presented as . ; .

(a)

(b)

(c)

(d)

(e)