Robust Plasma Cell Response to Skin-Inoculated Dengue Virus in Mice

Figure 4

PC distribution and proliferative state during DENV infection. (a) DLN cryosections analyzed at days 7, 10, 14, and 21 postcutaneous inoculation with DENV (left panel) or iDENV (right panel). CD138 (green) antibody-labelled PCs, antibodies for IgD (white), and Ki-67 (red) depict GC zones and CD4 (blue) for T zone (T). Panels on the right of each group correspond to the white squares indicated on the left. PC main location is indicated with purple arrowheads for each time point. Cell suspensions from DLNs were colabelled with antibodies to surface CD138 and Ly6C and intracellularly for Ki-67. (b) Representative flow cytometry contour plots of proliferating Ki-67⁺ PCs at day 3 p.i. Kinetic of the proportion (c) and numbers (d) of Ki-67⁺ PCs in the DLN during the kinetic. Data shown represent the and are representative of four independent experiments with two mice per group per time point and histology results from two experiments with four mice per group per time point. Two-way ANOVA with the Bonferroni post hoc test were used for the statistical analysis. , , , and .

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