Robust Plasma Cell Response to Skin-Inoculated Dengue Virus in Mice

Figure 5

DENV cutaneous infection induces a rapid entrance of PCs to cell cycle and a low apoptosis ratio. For cell cycle analysis by flow cytometry, cell suspensions from DLNs were colabelled with antibodies to surface CD138 and Ly6C and intranuclearly for Ki-67. The DNA was stained with Hoechst 33258. (a) Representative cell cycle gating analysis on PCs from DLN of mice 7 d p.i. with DENV or controls. (b) Percentage of PCs in each cell cycle phase during the kinetic in the DLN of mice postcutaneous inoculation with DENV, iDENV, or endotoxin-free PBS. (c) Representative cell cycle gating strategy on PCs (CD138⁺Ly6C⁺) from a DLN of mice cutaneously inoculated with DENV emphasizing the sub-G0/apoptotic population (red box). (d) Proportion of sub-G0/apoptotic PCs during the kinetic. (e) Proportion of apoptotic PCs expressing the active form of caspase-3 during the kinetic. Red dotted lines in (d) and (e) represent the mean levels in the PBS group. Data shown represent the and are representative of four independent experiments with two mice per group per time point.

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