Recovery from Liver Failure and Fibrosis in a Rat Portacaval Anastomosis Model after Neurointermediate Pituitary Lobectomy

Figure 9

Liver slides stained with Sirius Red analyzed under polarized light to assess the area of fibrosis. (a) Periportal area from an IC animal. Normal distribution of type III collagen (mainly green color) is observed. (b) NIL liver slide. Similar distribution of collagens of the IC group is apparent. (c) PCA liver slide. Increase in collagen type I (red) and III (yellow) distribution is observed in the periportal area, with thickening of the triad vessels. Also, an increased collagen invasion into the surrounding liver parenchyma () is apparent. (d) PCA+NIL. It shows regression of type I collagen, mainly in the periportal area (). (e) Comparisons of percentage of the fibrosis areas in the several experimental groups. Observe that between the IC and NIL groups, similar percentages of fibrosis are apparent. In contrast, the PCA animals developed a significant increase in the fibrotic area (: IC vs. PCA). In the PCA+NIL group, the percentage of fibrosis was significantly decreased as compared with the PCA group (: PCA vs. PCA+NIL group), indicating reversible effect of AVP deficiency on liver fibrosis. It was evaluated with analysis of variance test with the Tukey post hoc values, which are expressed as the . Pictures are taken at 20x magnification.

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