Research Article

Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates

Figure 3

Expression and purification of HIV-1 Nef protein in Rosetta: lane 1: before induction; lane 2: 5 hours after induction with IPTG (1 mM); lane 3: 16 hours after induction with IPTG (1 mM); lane 4: the purified Nef protein; MW: molecular weight marker (prestained protein ladder, 10-180 kDa, Fermentas).