Therapeutic Mechanism and Effect of Camptothecin on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice

Figure 6

Effects of camptothecin (CPT) on LPS-induced inflammatory response in RAW264.7 cells. RAW264.7 cells were cultured with different doses of CPT (0.1, 0.2, 0.4, 0.8, 1.0, 2.0, and 5.0 μmol/mL) for 4 h, and their viability was determined by CCK8 assay. (a) The effect of CPT was determined by CCK8 assay. RAW264.7 cells were pretreated with different doses of CPT (0.25, 0.5, and 1 μmol/mL) for 1 h and stimulated with LPS for 4 h. Protein and mRNA levels were determined by qRT-PCR and western blot, respectively. The mRNA levels of (b) IL-1β, (c) IL-6, (d) TNF-α, (e) iNOS, and (f) COX-2 were determined and normalized to the mRNA level of β-actin. The protein levels of (g, i) COX-2 and (g, h) iNOS and the relative protein levels were quantified by scanning densitometry and normalized to the protein level of β-actin. Values are presented as (, , , and vs. the LPS group).

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