Therapeutic Mechanism and Effect of Camptothecin on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice

Figure 7

Effects of camptothecin (CPT) on LPS-induced activation of the AKT/NF-κB signaling pathway in RAW264.7 cells. Total protein in RAW264.7 cells was collected following 4 h of LPS stimulation. CPT was added 1 h before LPS stimulation. Protein levels of p-AKT and p-IκBα were detected by western blot and quantitatively assessed via densitometry using (a, b) AKT and (a, c) IκBα as internal controls. Protein levels were measured using ImageJ (http://imagej.nih.gov/ij/) and normalized to the protein level of AKT and IκBα. Values are presented as ( vs. LPS, vs. LPS).

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