Norepinephrine Enhances Aerobic Glycolysis and May Act as a Predictive Factor for Immunotherapy in Gastric Cancer

Figure 4

NE expression promotes tumor cell glycolysis. Regarding the gastric cancer cell line BGC-823, the Pkm, Pfkl, Ldha, Eno1, and Pgam2 mRNA expression levels were significantly elevated in the NE treatment group compared with the PBS treatment group. Regarding the other HGC-27 cancer cell line, the Pkm, Pfkl, Ldha, and Eno1 mRNA expression levels were significantly elevated in the NE treatment group compared with the PBS treatment group (a). After NE treatment, the EACR increased obviously for BGC-823 cells compared with PBS treatment. Regarding glycolysis and the glycolytic capacity, the NE and PBS treatment groups showed significant differences (b). After NE treatment, the OCR decreased for BGC-823 cells compared with that after PBS treatment. Regarding ATP production and maximal respiration, the NE and PBS treatment groups showed significant differences (c). In both BGC-823 and HGC-27 cells, lactate production and glucose uptake capability were more enhanced in the NE treatment group than in the PBS treatment group (d, e).

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