Bone Marrow Neutrophils of Multiple Myeloma Patients Exhibit Myeloid-Derived Suppressor Cell Activity

Figure 2

Inhibition of T-cell proliferation and IFN-γ production by bone marrow NDG. (a) Median proliferation for each group is 94% (95% CI: 88–95), 91% (95% CI: 82–93), and 71 (95% CI: 50–79), respectively. The proliferation of T-cells was not inhibited by HD NDG () but by NDG from MM patients (). (b) The median IFN-γ production for each group is 9700 pg/ml (95% CI: 8200–13500), 3500 pg/ml (95% CI: 1450–7800), and 1600 (95% CI: 600–4500), respectively. Both HD NDG () and MM NDG () have the ability to inhibit IFN-γ production. (c) The effect of HD NDG on T-cell proliferation () differed between +2% and -26% proliferation. (d) Inhibition by NDGs from MM patients () differed between 1.7 and 60%. Error bars indicate median with range. Statistical significance was tested with Wilcoxon matched pairs signed rank test. Normal density neutrophils become activated by T-cells over time.

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